biopsies. The cell selection process directly
aligns with the company’s experience in
additive manufacturing, precisely placing
material where it belongs.
Parker worked with Dr. Muschler
and his team, including medical institutions and Biospherix ( www.biospher-ix.com), provider of the Cytocentric
platform, to develop the Cell X device.
Cell X combines LFOV imaging and
control capabilities with precision
instrumentation, fluidics, and documentation. Cell X opens new dimensions of opportunity for discovery and
development of stem cell therapeutics.
These same features also provide new
avenues for quantitative personalized
cell-based diagnostics, optimization of
biomaterials and bioactive surface design, and drug discovery applications.
The central core of the device is a high
quality automated inverted microscope
and CCD camera, with both brightfield
and fluorescent imaging options.
Other enabling features added to this
standard laboratory equipment are:
High precision low profile XY system
— This positions the cell carrying trays
in the optical system with submicron
repeatability (0.1µm). Integrated high
resolution feedback devices allow the
robot to precisely determine the exact
position of cells. Custom trays allow
the use of any standard cell culture
plate or dish, as well as options for
automatic loading and unloading of
plates. The low profile of this design
was key for keeping optics in the focal
range of the microplates.
Rigid machine base — This base was
custom engineered to align the microscope and positioning axes. This
structure provides a very stiff, stable platform with integrated isolating pads to
minimize the vibration the robot might
see from external sources.
Light filter adjustment axis — A high
precision miniature ballscrew-driven axis
used to adjust filters automatically to
optimize the lighting of the cells.
Tip load and remove station — Dispo-
sable tips used in picking cells of interest
are automatically loaded and removed
without human intervention.
Z-axis positioners — Three Z axes enable
the picking and placing of cells or fluids.
The first axis uses Parker’s Smart Syringe
pump to aspirate the cells of interest.
The second axis is used for precise media
removal around the area of interest. The
third axis allows for additional steps for
multiple fluids or reagents.
Cell optimized ecosystem and cell technician optimized work space — Cytocentric
platform complements machine by
neutralizing typical negative side effects
of machine on cells (particles, heat,
vibration, etc.), and synergizes with machine by optimizing all the critical cell
process parameters (temperature, hu-
Parker’s mSR Series compact linear
positioners combine smooth motion with
sub-micron level precision for ±0.1µm
Global Manufacturing For Single Use
And Disposable Medical Devices